Semi-Quantitative PCR for Quantification of Hepatotoxic Cyanobacteria

Semi-Quantitative PCR for Quantification of Hepatotoxic Cyanobacteria

Blooms of microcystin-producing cyanobacteria are a problem worldwide. Microcystin is a liver hepatotoxin commonly found in bodies of water and is produced mainly by the genus Microcystis. The aim of the present study was to develop and assess a competitive PCR method for the quantification of toxic and non-toxic Microcystis cells using the cpcBA and mcyB genes, which are respectively involved ...

Quantitative or semi-quantitative PCR: reality versus myth.

Johannes Bos University of Utrecht David Botstein Stanford University School of Medicine Jeff Chamberlain University of Michigan Medical School Henry Erlich Roche Molecular Systems Kenshi Hayashi National Cancer Research Institute, Tokyo Bernhard Horsthemke University of Essen Pieter de Jong Lawrence Livermore National Laboratory David Kemp Menzies School of Health Research Kenneth Kidd Yale Un...

A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data

BACKGROUND Quantitative PCR (qPCR) is a workhorse laboratory technique for measuring the concentration of a target DNA sequence with high accuracy over a wide dynamic range. The gold standard method for estimating DNA concentrations via qPCR is quantification cycle () standard curve quantification, which requires the time- and labor-intensive construction of a standard curve. In theory, the sha...

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)